HNF4A monoclonal antibody (M04), clone 4E2
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More Files
- More Functions
Western Blot (Cell lysate)
HNF4A monoclonal antibody (M04), clone 4E2 Western Blot analysis of HNF4A expression in HepG2 ( Cat # L019V1 ).
Western Blot (Cell lysate)
HNF4A monoclonal antibody (M04), clone 4E2. Western Blot analysis of HNF4A expression in Jurkat ( Cat # L017V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.
Lane 1: HNF4A transfected lysate(51.6 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi ( Cat # H00003172-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody (M04), clone 4E2 (Cat # H00003172-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HNF4A.
Immunogen
HNF4A (NP_000448, 324 a.a. ~ 423 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRP
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HNF4A monoclonal antibody (M04), clone 4E2 Western Blot analysis of HNF4A expression in HepG2 ( Cat # L019V1 ).Western Blot (Cell lysate)
HNF4A monoclonal antibody (M04), clone 4E2. Western Blot analysis of HNF4A expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.
Lane 1: HNF4A transfected lysate(51.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi ( Cat # H00003172-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody (M04), clone 4E2 (Cat # H00003172-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HNF4A
Entrez GeneID
3172GeneBank Accession#
NM_000457Protein Accession#
NP_000448Gene Name
HNF4A
Gene Alias
FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14
Gene Description
hepatocyte nuclear factor 4, alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
HNF4-alpha|OTTHUMP00000031060|OTTHUMP00000031062|hepatic nuclear factor 4 alpha|hepatocyte nuclear factor 4 alpha|transcription factor-14
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Interactome
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Pathway
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Disease
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Publication Reference
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Efficient programming of human mesenchymal stem cell-derived hepatocytes by epigenetic regulations.
Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC.
Journal of Gastroenterology and Hepatology 2016 May; 32(1):261.
Application：IF, WB, Human, MSCs.
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Efficient programming of human mesenchymal stem cell-derived hepatocytes by epigenetic regulations.
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