HGD monoclonal antibody (M10), clone 3G4
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HGD.
Immunogen
HGD (NP_000178, 377 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
CFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLDENYHKCWEPLKSHFTPNSRNPAEPN
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HGD is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HGD on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — HGD
Entrez GeneID
3081GeneBank Accession#
NM_000187Protein Accession#
NP_000178Gene Name
HGD
Gene Alias
AKU, HGO
Gene Description
homogentisate 1,2-dioxygenase (homogentisate oxidase)
Gene Ontology
HyperlinkGene Summary
Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26. [provided by RefSeq
Other Designations
homogentisate 1,2-dioxygenase|homogentisicase
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