GUCY1A3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GUCY1A3 transfected lysate.
Western Blot
Lane 1: GUCY1A3 transfected lysate ( 76.01 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GUCY1A3 full-length
Host
Human
Theoretical MW (kDa)
76.01
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GUCY1A3 antibody (H00002982-B01) by Western Blots.
SDS-PAGE Gel
GUCY1A3 transfected lysate.
Western Blot
Lane 1: GUCY1A3 transfected lysate ( 76.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GUCY1A3
Entrez GeneID
2982GeneBank Accession#
BC028384Protein Accession#
AAH28384Gene Name
GUCY1A3
Gene Alias
GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1
Gene Description
guanylate cyclase 1, soluble, alpha 3
Omim ID
139396Gene Ontology
HyperlinkGene Summary
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit, such as alpha-1 (GUCY1A3), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).[supplied by OMIM
Other Designations
GC-S-alpha-1|soluble guanylate cyclase large subunit
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