PDIA3 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of PDIA3 transfected lysate using rabbit polyclonal anti-PDIA3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-PDIA3.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93%); Rat (92%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of PDIA3 transfected lysate using rabbit polyclonal anti-PDIA3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-PDIA3.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-PDIA3 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-PDIA3 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — PDIA3
Entrez GeneID
2923Gene Name
PDIA3
Gene Alias
ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Gene Description
protein disulfide isomerase family A, member 3
Omim ID
602046Gene Ontology
HyperlinkGene Summary
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq
Other Designations
58 kDa microsomal protein|OTTHUMP00000041709|endoplasmic reticulum P58|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3|protein disulfide-isomerase A3
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Interactome
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Pathway
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Disease
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