FRG1 monoclonal antibody (M01), clone 4A5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FRG1.
Immunogen
FRG1 (AAH53997, 1 a.a. ~ 258 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEYSYVKSTKLVLKGTKTKSKKKKSKDKKRKREEDEETQLDIVGIWWTVTNFGEISGTIAIEMDKGTYIHALDNGLFTLGAPHKEVDEGPSPPEQFTAVKLSDSRIALKSGYGKYLGINSDGLVVGRSDAIGPREQWEPVFQNGKMALLASNSCFIRCNEAGDIEAKSKTAGEEEMIKIRSCAERETKKKDDIPEEDKGNVKQCEINYVKKFQSFQDHKLKISKEDSKILKKARKDGFLHETLLDRRAKLKADRYCK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.12 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FRG1 monoclonal antibody (M01), clone 4A5 Western Blot analysis of FRG1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of FRG1 expression in transfected 293T cell line by FRG1 monoclonal antibody (M01), clone 4A5.
Lane 1: FRG1 transfected lysate(29 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of FRG1 transfected lysate using anti-FRG1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FRG1 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to FRG1 on HeLa cell. [antibody concentration 35 ug/ml] -
Gene Info — FRG1
Entrez GeneID
2483GeneBank Accession#
BC053997Protein Accession#
AAH53997Gene Name
FRG1
Gene Alias
FRG1A, FSG1
Gene Description
FSHD region gene 1
Omim ID
601278Gene Ontology
HyperlinkGene Summary
This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq
Other Designations
facioscapulohumeral muscular dystrophy region gene-1
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Interactome
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Publication Reference
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RNA Interference Improves Myopathic Phenotypes in Mice Over-expressing FSHD Region Gene 1 (FRG1).
Wallace LM, Garwick-Coppens SE, Tupler R, Harper SQ.
Mol Ther 2011 Jul; 19:2048.
Application:WB-Tr, WB-Ti, Human, Mouse, HEK293 cells, Muscles.
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RNA Interference Improves Myopathic Phenotypes in Mice Over-expressing FSHD Region Gene 1 (FRG1).
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