CLPS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CLPS transfected lysate.
Western Blot
Lane 1: CLPS transfected lysate ( 12.43 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CLPS full-length
Host
Human
Theoretical MW (kDa)
12.43
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CLPS antibody (H00001208-B01) by Western Blots.
SDS-PAGE Gel
CLPS transfected lysate.
Western Blot
Lane 1: CLPS transfected lysate ( 12.43 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CLPS
Entrez GeneID
1208GeneBank Accession#
NM_001832.2Protein Accession#
-Gene Name
CLPS
Gene Alias
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Gene Description
colipase, pancreatic
Omim ID
120105Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. [provided by RefSeq
Other Designations
OTTHUMP00000016271|colipase|pancreatic colipase preproprotein
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Interactome
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Disease
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