CENPB purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Cell lysate)
CENPB MaxPab polyclonal antibody. Western Blot analysis of CENPB expression in A-431.
Western Blot (Transfected lysate)
Western Blot analysis of CENPB expression in transfected 293T cell line (H00001059-T01) by CENPB MaxPab polyclonal antibody.
Lane1:CENPB transfected lysate(65.89 KDa).
Lane2:Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to CENPB on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human CENPB protein.
Immunogen
CENPB (AAH53847.1, 1 a.a. ~ 599 a.a) full-length human protein.
Sequence
MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CENPB MaxPab polyclonal antibody. Western Blot analysis of CENPB expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of CENPB expression in transfected 293T cell line (H00001059-T01) by CENPB MaxPab polyclonal antibody.
Lane1:CENPB transfected lysate(65.89 KDa).
Lane2:Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to CENPB on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — CENPB
Entrez GeneID
1059GeneBank Accession#
NM_001810Protein Accession#
AAH53847.1Gene Name
CENPB
Gene Alias
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Gene Description
centromere protein B, 80kDa
Omim ID
117140Gene Ontology
HyperlinkGene Summary
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq
Other Designations
OTTHUMP00000030132|centromere autoantigen B|centromere protein B|centromere protein B (80kD)
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Interactome
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