CENPB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CENPB transfected lysate.
Western Blot
Lane 1: CENPB transfected lysate ( 66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENPB full-length
Host
Human
Theoretical MW (kDa)
66
Interspecies Antigen Sequence
Mouse (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENPB antibody (H00001059-B01) by Western Blots.
SDS-PAGE Gel
CENPB transfected lysate.
Western Blot
Lane 1: CENPB transfected lysate ( 66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CENPB
Entrez GeneID
1059GeneBank Accession#
NM_001810Protein Accession#
NP_001801Gene Name
CENPB
Gene Alias
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Gene Description
centromere protein B, 80kDa
Omim ID
117140Gene Ontology
HyperlinkGene Summary
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq
Other Designations
OTTHUMP00000030132|centromere autoantigen B|centromere protein B|centromere protein B (80kD)
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