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CENPB 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00001059-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-CENPB full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 66
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CENPB antibody (H00001059-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00001059-T01
    CENPB transfected lysate.
    Western Blot
    QC Testing of H00001059-T01
    Lane 1: CENPB transfected lysate ( 66 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 1059
  • Gene Name:
  • CENPB
  • Gene Alias:
  • -
  • Gene Description:
  • centromere protein B, 80kDa
  • Gene Summary:
  • This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030132,centromere autoantigen B,centromere protein B,centromere protein B (80kD)
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