CD58 monoclonal antibody (M02), clone 3B12-D5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CD58.
Immunogen
CD58 (AAH05930, 1 a.a. ~ 240 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGMYAF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.14 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CD58 expression in transfected 293T cell line by CD58 monoclonal antibody (M02), clone 3B12-D5.
Lane 1: CD58 transfected lysate (Predicted MW: 28.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CD58 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — CD58
Entrez GeneID
965GeneBank Accession#
BC005930Protein Accession#
AAH05930Gene Name
CD58
Gene Alias
LFA-3, LFA3
Gene Description
CD58 molecule
Omim ID
153420Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
CD58 antigen, (lymphocyte function-associated antigen 3)|OTTHUMP00000024363
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Interactome
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Pathway
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Disease
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