CA12 monoclonal antibody (M01), clone 1D4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CA12 expression in transfected 293T cell line by CA12 monoclonal antibody (M01), clone 1D4.
Lane 1: CA12 transfected lysate(39.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CA12 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of CA12 over-expressed 293 cell line, cotransfected with CA12 Validated Chimera RNAi ( Cat # H00000771-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CA12 monoclonal antibody (M01), clone 1D4 (Cat # H00000771-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CA12.
Immunogen
CA12 (NP_996808, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CA12 expression in transfected 293T cell line by CA12 monoclonal antibody (M01), clone 1D4.
Lane 1: CA12 transfected lysate(39.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CA12 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CA12 over-expressed 293 cell line, cotransfected with CA12 Validated Chimera RNAi ( Cat # H00000771-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CA12 monoclonal antibody (M01), clone 1D4 (Cat # H00000771-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CA12
Entrez GeneID
771GeneBank Accession#
NM_206925Protein Accession#
NP_996808Gene Name
CA12
Gene Alias
CAXII, FLJ20151, HsT18816
Gene Description
carbonic anhydrase XII
Omim ID
603263Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
carbonic dehydratase
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Interactome
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Pathway
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