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Last updated: 2020/10/18
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APC monoclonal antibody (M01), clone 3E2 

  • Catalog # : H00000324-M01
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant APC.
  • Immunogen:
  • APC (NP_000029, 2744 a.a. ~ 2843 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • NPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00000324-M01
    Western Blot detection against Immunogen (36.85 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Interspecies Antigen Sequence:
  • Mouse (90%); Rat (89%)
  • Applications
  • Immunofluorescence
  • Immunofluorescence enlargeenlarge this image
  • Immunofluorescence of monoclonal antibody to APC on HeLa cell . [antibody concentration 10 ug/ml]
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Proximity Ligation Analysis of protein-protein interactions between JUP and APC. HeLa cells were stained with anti-JUP rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • Western Blot (Recombinant protein)
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 324
  • Gene Name:
  • APC
  • Gene Alias:
  • BTPS2,DP2,DP2.5,DP3,GS
  • Gene Description:
  • adenomatous polyposis coli
  • Gene Summary:
  • This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq
  • Other Designations:
  • adenomatosis polyposis coli tumor suppressor
  • Interactome
  • Interactome
  • Related Disease
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