ANXA2 monoclonal antibody (M02), clone 1G7
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More Files
- More Functions
Western Blot (Cell lysate)
ANXA2 monoclonal antibody (M02), clone 1G7 Western Blot analysis of ANXA2 expression in HepG2 ( Cat # L019V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.
Lane 1: ANXA2 transfected lysate(40.4 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ANXA2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (63.03 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ANXA2.
Immunogen
ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.03 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ANXA2 monoclonal antibody (M02), clone 1G7 Western Blot analysis of ANXA2 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.
Lane 1: ANXA2 transfected lysate(40.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to ANXA2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ANXA2
Entrez GeneID
302GeneBank Accession#
BC066955Protein Accession#
AAH66955Gene Name
ANXA2
Gene Alias
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV
Gene Description
annexin A2
Omim ID
151740Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000163650|annexin II|calpactin I heavy polypeptide|chromobindin 8|lipocortin II|placental anticoagulant protein IV
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Interactome
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Disease
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Publication Reference
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Protein nitration profile of CD3+ lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection.
Tramutola A, Abate G, Lanzillotta C, Triani F, Barone E, Iavarone F, Vincenzoni F, Castagnola M, Marziano M, Memo M, Garrafa E, Butterfield DA, Perluigi M, Domenico FD, Uberti D.
Free Radical Biology & Medicine 2018 Dec; 129:430.
Application：IP, WB, Human, Human blood.
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The Metalloprotease, Mpr1, Engages AnnexinA2 to Promote the Transcytosis of Fungal Cells across the Blood-Brain Barrier.
Na Pombejra S, Salemi M, Phinney BS, Gelli A.
Frontiers in Cellular and Infection Microbiology 2017 Jun; 7:296.
Application：IF, Human, Human brain microvascular endothelial cells.
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The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization.
Zakrzewicz D, Didiasova M, Zakrzewicz A, Hocke AC, Uhle F, Markart P, Preissner KT, Wygrecka M.
The Biochemical Journal 2014 Jun; 460(2):295.
Application：Flow Cyt, WB-Ce, Human, MDA-MB-231 cell.
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Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans.
Deng FY, Lei SF, Zhang Y, Zhang YL, Zheng YP, Zhang LS, Pan R, Wang L, Tian Q, Shen H, Zhao M, Lundberg YW, Liu YZ, Papasian CJ, Deng HW.
Molecular & Cellular proteomics: MCP 2011 Nov; 10(11):M111.01170.
Application：WB-Ce, Human, Peripheral blood monocytes.
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Proteomic analysis of climatic keratopathy droplets.
Menegay M, Lee D, Tabbara KF, Cafaro TA, Urrets-Zavalia JA, Serra HM, Bhattacharya SK.
Investigative Ophthalmology & Visual Science 2008 Mar; 49(7):2829.
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Protein nitration profile of CD3+ lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection.
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