ANXA2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
ANXA2 polyclonal antibody (Cat # PAB6109) staining (1 ug/mL) of A-431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ANXA2.
Immunogen
A synthetic peptide corresponding to human ANXA2.
Sequence
STVHEILCKLSLEGD
Host
Goat
Theoretical MW (kDa)
40.4, 38.6
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms. NP_004030.1 and NP_001002857.1 are varients of isoform 2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ANXA2 polyclonal antibody (Cat # PAB6109) staining (1 ug/mL) of A-431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ANXA2
Entrez GeneID
302Protein Accession#
NP_001002858.1;NP_004030.1;NP_001002857.1Gene Name
ANXA2
Gene Alias
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV
Gene Description
annexin A2
Omim ID
151740Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000163650|annexin II|calpactin I heavy polypeptide|chromobindin 8|lipocortin II|placental anticoagulant protein IV
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Interactome
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Disease
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Publication Reference
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Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption.
Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD.
The Journal of Biological Chemistry 1994 Nov; 269(46):28696.
Application：WB-Ce, WB-Tr, Human, 293, Osteoclast-like giant cells.
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Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption.
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