ALAS1 monoclonal antibody (M03), clone 2D5
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant ALAS1.
Immunogen
ALAS1 (NP_000679, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MESVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRALSTAAVHYQQIKETPPASEKDKTAKAKVQQTPDGSQQSPDGTQLPSGHPLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (83)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ALAS1 monoclonal antibody (M03), clone 2D5. Western Blot analysis of ALAS1 expression in COLO 320 HSR ( Cat # L020V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ALAS1 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — ALAS1
Entrez GeneID
211GeneBank Accession#
NM_000688Protein Accession#
NP_000679Gene Name
ALAS1
Gene Alias
ALAS, ALAS3, ALASH, MIG4
Gene Description
aminolevulinate, delta-, synthase 1
Omim ID
125290Gene Ontology
HyperlinkGene Summary
Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM
Other Designations
aminolevulinate, delta, synthase 1|migration-inducing protein 4
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Interactomes
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Pathways
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Diseases
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