ALAS1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against ALAS1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant ALAS1.
Theoretical MW (kDa)
71
Reactivity
Human
Specificity
This antibody detects endogenous levels of ALAS and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:JAR whole cell lysate Lane2:HUVEC whole cell lysate Lane3:Hela whole cell lysate with ALAS1 recombinant monoclonal antibody (Cat # RAB02667) at 1:1000 dilution.Immunocytochemistry
Immunocytochemical staining of HeLa cells using ALAS1 recombinant monoclonal antibody (Cat # RAB02667)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — ALAS1
Entrez GeneID
211Protein Accession#
P13196Gene Name
ALAS1
Gene Alias
ALAS, ALAS3, ALASH, MIG4
Gene Description
aminolevulinate, delta-, synthase 1
Omim ID
125290Gene Ontology
HyperlinkGene Summary
Delta-aminolevulinate synthase (ALAS; EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2 (MIM 301300).[supplied by OMIM
Other Designations
aminolevulinate, delta, synthase 1|migration-inducing protein 4
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