NES/CEN1p FISH Probe
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Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lung squamous cell carcinoma (FFPE) stained with NES/CEN1p FISH Probe. Human lung squamous cell carcinoma showed no NES gene amplification.
Hybridization position of the probes on the chromosome:
Hybridization position of the probes on the chromosome:
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Specification
Product Description
Labeled FISH probes for identification of gene amplification using Fluorescent In Situ Hybridization Technique. (Technology).
Probe 1
Name: NES
Size: Approximately 290kb
Fluorophore: Texas Red
Location: 1p23.1Probe 2
Name: CEN1p
Size: Approximately 780kb
Fluorophore: FITC
Location: 1p13.3Probe Gap
The gap between two probes is approximately 45,860 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome:
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Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lung squamous cell carcinoma (FFPE) stained with NES/CEN1p FISH Probe. Human lung squamous cell carcinoma showed no NES gene amplification. -
Gene Info — NES
Entrez GeneID
10763Gene Name
NES
Gene Alias
FLJ21841, Nbla00170
Gene Description
nestin
Omim ID
600915Gene Ontology
HyperlinkGene Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
Other Designations
OTTHUMP00000032198
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