PIK3CA/CEN3q FISH Probe
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Specification
Product Description
Labeled FISH probes for identification of gene amplification using Fluorescent In Situ Hybridization Technique. (Technology).
Probe 1
Name: PIK3CA
Size: Approximately 280kb
Fluorophore: Texas Red
Location: 3q26.32Probe 2
Name: CEN3q
Size: Approximately 500kb
Fluorophore: FITC
Location: 3q12.1Probe Gap
The gap between two probes is approximately 82,800 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome:
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Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lung adenosquamous cell carcinoma (FFPE) stained with PIK3CA/CEN3q FISH Probe. Human lung adenosquamous cell carcinoma showed PIK3CA gene amplification. -
Gene Info — PIK3CA
Entrez GeneID
5290Gene Name
PIK3CA
Gene Alias
MGC142161, MGC142163, PI3K, p110-alpha
Gene Description
phosphoinositide-3-kinase, catalytic, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
Other Designations
PI3-kinase p110 subunit alpha|PtdIns-3-kinase p110|phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha|phosphatidylinositol 3-kinase, catalytic, alpha polypeptide|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
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Interactome
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Pathway
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Disease
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Publication Reference
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PIK3CA Amplification Associates with Aggressive Phenotype but Not Markers of AKT-MTOR Signaling in Endometrial Carcinoma.
Holst F, Werner HMJ, Mjøs S, Hoivik EA, Kusonmano K, Wik E, Berg A, Birkeland E, Gibson WJ, Halle MK, Trovik J, Cherniack AD, Kalland KH, Mills GB, Singer CF, Krakstad C, Beroukhim R, Salvesen HB.
Clinical Cancer Research 2019 Jan; 25(1):334.
Application:FISH, Human, Human endometrial tumor samples.
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PIK3CA mutation in Chinese patients with lung squamous cell carcinoma.
Yu J, Bai H, Wang Z, Wei Z, Ding X, Duan J, Yang L, Wu M, Wang Y, Wang J.
Chinese Journal of Cancer Research 2013 Aug; 25(4):416.
Application:FISH, Human, Lung squamous cell carcinoma.
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PIK3CA Amplification Associates with Aggressive Phenotype but Not Markers of AKT-MTOR Signaling in Endometrial Carcinoma.
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