RAF1 (phospho S259) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S259 of human RAF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of RAF1 (phospho S259) polyclonal antibody (Cat # PAB8117) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — RAF1
Entrez GeneID
5894Protein Accession#
NP_002871;P04049Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP.
Molecular and Cellular Biology 2004 Nov; 24(21):9527.
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Raf-1 activation disrupts its binding to keratins during cell stress.
Ku NO, Fu H, Omary MB.
Journal of Cellular Biology 2004 Aug; 166(4):479.
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Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.
Gohlke H, Kuhn LA, Case DA.
Proteins 2004 Aug; 56(2):322.
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Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
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