PPID polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PPID polyclonal antibody (Cat # PAB7312) (0.1 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing PPID and probed with PPID polyclonal antibody (Cat # PAB7312) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPID.
Immunogen
A synthetic peptide corresponding to human PPID.
Sequence
C-DENFHYKHDREG
Host
Goat
Theoretical MW (kDa)
40.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPID polyclonal antibody (Cat # PAB7312) (0.1 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing PPID and probed with PPID polyclonal antibody (Cat # PAB7312) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPID
Entrez GeneID
5481Protein Accession#
NP_005029.1Gene Name
PPID
Gene Alias
CYP-40, CYPD, MGC33096
Gene Description
peptidylprolyl isomerase D
Omim ID
601753Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq
Other Designations
40 kDa peptidyl-prolyl cis-trans isomerase D|PPIase|cyclophilin 40|cyclophilin D|cyclophilin-related protein|rotamase
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Interactome
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Pathway
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Publication Reference
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Cyclophilin D inactivation protects axons in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis.
Forte M, Gold BG, Marracci G, Chaudhary P, Basso E, Johnsen D, Yu X, Fowlkes J, Rahder M, Stem K, Bernardi P, Bourdette D.
PNAS 2007 May; 104(18):7558.
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Cyclophilin D inactivation protects axons in experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis.
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