POU5F1 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide corresponding to human POU5F1.
Sequence
C-VTTLGSPMHSN
Host
Goat
Theoretical MW (kDa)
38.6, 30.1
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_002692 and NP_9760.34)
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POU5F1 polyclonal antibody (Cat # PAB6831) (1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU5F1
Entrez GeneID
5460Protein Accession#
NP_002692.2;NP_9760.34.2Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
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Interactomes
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Diseases
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Publication Reference
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OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma.
Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L.
The American Journal of Surgical Pathology 2004 Jul; 28(7):935.
Application:IHC-P, Human, Human testicular tumors.
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OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma.
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