ATF4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATF4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S245 of human ATF4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ATF4 (arrow) using rabbit ATF4 polyclonal antibody (Cat # PAB4874). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATF4
Entrez GeneID
468Protein Accession#
NP_001666;Q96AQ3Gene Name
ATF4
Gene Alias
CREB-2, CREB2, TAXREB67, TXREB
Gene Description
activating transcription factor 4 (tax-responsive enhancer element B67)
Omim ID
604064Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq
Other Designations
activating transcription factor 4|cAMP response element-binding protein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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TRB3 inhibits the transcriptional activation of stress-regulated genes by a negative feedback on the ATF4 pathway.
Jousse C, Deval C, Maurin AC, Parry L, Cherasse Y, Chaveroux C, Lefloch R, Lenormand P, Bruhat A, Fafournoux P.
The Journal of Biological Chemistry 2007 May; 282(21):15851.
Application:ChIP, WB, Human, Mouse , HeLa cells, MEFs.
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ATF4 differentially regulates transcriptional activation of myeloid-specific genes by C/EBPepsilon and C/EBPalpha.
Gombart AF, Grewal J, Koeffler HP.
Journal of Leukocyte Biology 2007 Mar; 81(6):1535.
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Association analysis of ATF4 and ATF5, genes for interacting-proteins of DISC1, in bipolar disorder.
Kakiuchi C, Ishiwata M, Nanko S, Kunugi H, Minabe Y, Nakamura K, Mori N, Fujii K, Yamada K, Yoshikawa T, Kato T.
Neuroscience Letters 2007 Feb; 417(3):316.
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TRB3 inhibits the transcriptional activation of stress-regulated genes by a negative feedback on the ATF4 pathway.
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