ATF4 recombinant monoclonal antibody, clone 9E1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: 293 whole cell lysate, Lane 4: HepG2 whole cell lysate, Lane 5: Jurkat whole cell lysate and Lane 6: K562 whole cell lysate with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:160).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:32.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349).
Lane 1: rabbit control IgG, Lane 2: RAB04349 precipitates and Lane 3: Input (Hela whole cell lysate).
Flow Cytometry
Flow cytometric analysis of HeLa cells with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATF4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATF4.
Theoretical MW (kDa)
Calculated MW: 39 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: 293 whole cell lysate, Lane 4: HepG2 whole cell lysate, Lane 5: Jurkat whole cell lysate and Lane 6: K562 whole cell lysate with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349).Immunohistochemistry
Immunohistochemical staining of human breast cancer with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:160).Immunofluorescence
Immunofluorescent staining of HepG2 cells with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:32.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349).
Lane 1: rabbit control IgG, Lane 2: RAB04349 precipitates and Lane 3: Input (Hela whole cell lysate).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with ATF4 recombinant monoclonal antibody, clone 9E1 (Cat # RAB04349) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — ATF4
Entrez GeneID
468Protein Accession#
P18848Gene Name
ATF4
Gene Alias
CREB-2, CREB2, TAXREB67, TXREB
Gene Description
activating transcription factor 4 (tax-responsive enhancer element B67)
Omim ID
604064Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq
Other Designations
activating transcription factor 4|cAMP response element-binding protein 2
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Interactome
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Pathway
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Disease
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