AKT1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human AKT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Y-79 cell lysate (35 ug/lane) with AKT1 polyclonal antibody (Cat # PAB4548).Western Blot (Transfected lysate)
Western blot analysis of AKT1 (arrow) using AKT1 polyclonal antibody (Cat # PAB4548).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
Powell DJ, Hajduch E, Kular G, Hundal HS.
Molecular and Cellular Biology 2003 Nov; 23(21):7794.
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Increase of AKT/PKB expression correlates with gleason pattern in human prostate cancer.
Liao Y, Grobholz R, Abel U, Trojan L, Michel MS, Angel P, Mayer D.
International Journal of Cancer 2003 Nov; 107(4):676.
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Akt activation disrupts mammary acinar architecture and enhances proliferation in an mTOR-dependent manner.
Debnath J, Walker SJ, Brugge JS.
Journal of Cellular Biology 2003 Oct; 163(2):315.
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
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