AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:293 whole cell lysate (treated with Calyculin A 100nM/60 mins) and Lane 2: A549 whole cell lysate (not treated) with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283).
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human lung cancer with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:100)
Immunofluorescence
Immunofluorescent staining of Hela cells with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:68). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of 293 cell lysate with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283).
Lane 1: rabbit control IgG, Lane 2: RAB04283 precipitates and Lane 3: Input (293 whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AKT1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human AKT1.
Theoretical MW (kDa)
Calculated MW: 60 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:293 whole cell lysate (treated with Calyculin A 100nM/60 mins) and Lane 2: A549 whole cell lysate (not treated) with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283).Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human lung cancer with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:100)Immunofluorescence
Immunofluorescent staining of Hela cells with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283) (diluated at 1:68). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of 293 cell lysate with AKT1 (phospho Ser473) recombinant monoclonal antibody, clone 4H12 (Cat # RAB04283).
Lane 1: rabbit control IgG, Lane 2: RAB04283 precipitates and Lane 3: Input (293 whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Protein Accession#
P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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