RCVRN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The RCVRN polyclonal antibody (Cat # PAB3609) is used in Western blot to detect Recoverin in Y-79 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RCVRN polyclonal antibody (Cat # PAB3609) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RCVRN.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RCVRN.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The RCVRN polyclonal antibody (Cat # PAB3609) is used in Western blot to detect Recoverin in Y-79 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RCVRN polyclonal antibody (Cat # PAB3609) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — RCVRN
Entrez GeneID
5957Protein Accession#
S43855Gene Name
RCVRN
Gene Alias
RCV1
Gene Description
recoverin
Omim ID
179618Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq
Other Designations
cancer associated retinopathy antigen
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Interactome
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Publication Reference
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Functional analysis of the human recoverin gene promoter.
Wiechmann A, Howard E.
Current Eye Research 2003 Jan; 26(1):25.
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Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines.
Matsubara S, Yamaji Y, Sato M, Fujita J, Takahara J.
British Journal of Cancer 1996 Nov; 74(9):1419.
Application：WB-Ce, Human, MN-1112 cells, Recombinant proteins.
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Characterization of a small-cell-lung-carcinoma cell line from a patient with cancer-associated retinopathy.
Yamaji Y, Matsubara S, Yamadori I, Sato M, Fujita T, Fujita J, Takahara J.
International Journal of Cancer 1996 Mar; 65(5):671.
Application：WB-Ce, Human, MN-1112, TK-130 cells.
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Functional analysis of the human recoverin gene promoter.
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