BBC3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BBC3.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human BBC3.
Host
Rabbit
Theoretical MW (kDa)
20.5
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat and mouse sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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Gene Info — BBC3
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Interactome
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Pathway
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Publication Reference
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PUMA couples the nuclear and cytoplasmic proapoptotic function of p53.
Chipuk JE, Bouchier-Hayes L, Kuwana T, Newmeyer DD, Green DR.
Science 2005 Sep; 309(5741):1732.
Application：WB, Mouse, MEFs.
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Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T.
PNAS 2001 Sep; 98(20):11318.
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PUMA induces the rapid apoptosis of colorectal cancer cells.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B.
Molecular Cell 2001 Mar; 7(3):673.
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PUMA couples the nuclear and cytoplasmic proapoptotic function of p53.
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