BBC3 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:K562 whole cell lysate with BBC3 recombinant monoclonal antibody (Cat # RAB02693) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BBC3 recombinant monoclonal antibody (Cat # RAB02693). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using BBC3 recombinant monoclonal antibody (Cat # RAB02693)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against BBC3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant BBC3.
Theoretical MW (kDa)
20
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PUMA and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:K562 whole cell lysate with BBC3 recombinant monoclonal antibody (Cat # RAB02693) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BBC3 recombinant monoclonal antibody (Cat # RAB02693). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using BBC3 recombinant monoclonal antibody (Cat # RAB02693)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — BBC3
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Interactome
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Pathway
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