The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12725) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane. A : PTEN (pS385) (Phosphospecific) B : PTEN (Paired S385) (Non phosphospeicfic) C : Non-related phosphospecific peptide were blotted at a 1 : 2000 dilution by : 1 : Phospho-PTEN S385 polyclonal antibody (Cat # PAB12751), 2 : PTEN polyclonal antibody (Cat # PAB12725).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
MMAC1 phosphatase and tensin homolog deleted on chromosome 10,OTTHUMP00000020032,mutated in multiple advanced cancers 1