TFEB polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of TFEB.
Immunogen
A synthetic peptide corresponding to internal region of human TFEB.
Sequence
C-QKDLQKSRELENH
Host
Goat
Theoretical MW (kDa)
52.9
Reactivity
Human
Specificity
This antibody is expected not to cross-react to TFE3 and MITF.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:1000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TFEB polyclonal antibody (Cat # PAB11591) (1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TFEB
Entrez GeneID
7942Protein Accession#
NP_009093.1Gene Name
TFEB
Gene Alias
AlphaTFEB, TCFEB, bHLHe35
Gene Description
transcription factor EB
Omim ID
600744Gene Ontology
HyperlinkOther Designations
OTTHUMP00000043540|OTTHUMP00000043542|OTTHUMP00000071327|T-cell transcription factor EB
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Interactomes
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Publication Reference
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Oncogenic MITF dysregulation in clear cell sarcoma: defining the MiT family of human cancers.
Davis IJ, Kim JJ, Ozsolak F, Widlund HR, Rozenblatt-Rosen O, Granter SR, Du J, Fletcher JA, Denny CT, Lessnick SL, Linehan WM, Kung AL, Fisher DE.
Cancer Cell 2006 Jun; 9(6):473.
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Oncogenic MITF dysregulation in clear cell sarcoma: defining the MiT family of human cancers.
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