Vegfa (Rat) Recombinant Protein
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Result of activity analysis
Result of activity analysis
Serial dilutions of rat Vegfa, starting at 250 ng/mL, were added to HUVECs. After 87 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50.
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Specification
Product Description
Rat Vegfa recombinant protein expressed in Escherichia coli.
Sequence
MAPTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
38.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent induced proliferation of HUVECs. The expected ED50 for this effect is 5.9-8.9 ng/mL.
Storage Buffer
Lyophilized from 10 mM NaP, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Vegfa
Entrez GeneID
83785Protein Accession#
AAL07526.1Gene Name
Vegfa
Gene Alias
VEGF164, Vegf
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkOther Designations
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