Vegfa (Rat) Recombinant Protein
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Specification
Product Description
Rat Vegfa recombinant protein expressed in Saccharomyces cerevisiae.
Sequence
MAPTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Host
Yeast
Theoretical MW (kDa)
25.7
Form
Lyophilized
Preparation Method
yeast expression system
Purification
chromatographic
Purity
> 90% as determined by SDS-PAGE.
Activity
ED50 is 0.75-3.75 ng/mL, measured in a cell proliferation assay using HUVEC cells.
Storage Buffer
Lyophilized from 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C for long term storage.
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Vegfa
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