UNG monoclonal antibody, clone k1C12
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant UNG.
Immunogen
Recombinant protein corresponding to full length human UNG.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: MCF7 cell lysate, Lane 4: K562 cell lysate, Lane 5: 293T cell lysate, Lane 6: HepG2 cell lysate, Lane 7: A549 cell lysate, Lane 8: SK-OV-3 cell lysate, Lane 9: PC3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — UNG
Entrez GeneID
7374GeneBank Accession#
NM_080911Protein Accession#
NP_550433Gene Name
UNG
Gene Alias
DGU, DKFZp781L1143, HIGM4, UDG, UNG1, UNG15, UNG2
Gene Description
uracil-DNA glycosylase
Gene Ontology
HyperlinkGene Summary
This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. [provided by RefSeq
Other Designations
uracil-DNA glycosylase 1, uracil-DNA glycosylase 2|uracil-DNA glycosylase 2
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Interactome
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Pathway
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Disease
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Publication Reference
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A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping.
Bellamy SR, Krusong K, Baldwin GS.
Nucleic Acids Research 2007 Feb; 35(5):1478.
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Characterization of the uracil-DNA glycosylase activity of Epstein-Barr virus BKRF3 and its role in lytic viral DNA replication.
Lu CC, Huang HT, Wang JT, Slupphaug G, Li TK, Wu MC, Chen YC, Lee CP, Chen MR.
Journal of Virology 2007 Feb; 81(3):1195.
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Analysis of uracil-DNA glycosylases from the murine Ung gene reveals differential expression in tissues and in embryonic development and a subcellular sorting pattern that differs from the human homologues.
Nilsen H, Steinsbekk KS, Otterlei M, Slupphaug G, Aas PA, Krokan HE.
Nucleic Acids Research 2000 Jun; 28(12):2277.
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A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping.
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