MAP2K6 monoclonal antibody, clone 3H12C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green). Blue: DRAQ5 fluorescent DNA dye. MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green). Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MAP2K6.
Immunogen
Recombinant protein corresponding to human MAP2K6.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green). Blue: DRAQ5 fluorescent DNA dye. MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794).Flow Cytometry
Flow cytometric analysis of HeLa cells using MAP2K6 monoclonal antibody, clone 3H12C8 (Cat # MAB10794) (green) and negative control (purple). -
Gene Info — MAP2K6
Entrez GeneID
5608Gene Name
MAP2K6
Gene Alias
MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3
Gene Description
mitogen-activated protein kinase kinase 6
Omim ID
601254Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
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Interactome
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Pathway
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Disease
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