SOD1 monoclonal antibody, clone 6F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) against HeLa (1), NIH/3T3 (2), A-549 (3) and A-431 (4) cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 (A) and SK-BR-3 (B) cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of A-431 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SOD1.
Immunogen
Recombinant protein corresponding to full length human SOD1.
Host
Mouse
Theoretical MW (kDa)
18
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) against HeLa (1), NIH/3T3 (2), A-549 (3) and A-431 (4) cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 (A) and SK-BR-3 (B) cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-431 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green) and negative control (purple). -
Gene Info — SOD1
Entrez GeneID
6647Gene Name
SOD1
Gene Alias
ALS, ALS1, IPOA, SOD, homodimer
Gene Description
superoxide dismutase 1, soluble
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations
Cu /Zn superoxide dismutase|Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
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Interactome
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Pathway
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Disease
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