Mouse monoclonal antibody raised against recombinant SOD1.
Immunogen:
Recombinant protein corresponding to human SOD1.
Host:
Mouse
Theoretical MW (kDa):
18
Reactivity:
Human, Mouse
Form:
Liquid
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) against HeLa (1), NIH/3T3 (2), A-549 (3) and A-431 (4) cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 (A) and SK-BR-3 (B) cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
ELISA
Flow Cytometry
Flow cytometric analysis of A-431 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green) and negative control (purple).
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations:
Cu /Zn superoxide dismutase,Cu/Zn superoxide dismutase,SOD, soluble,indophenoloxidase A,superoxide dismutase, cystolic