SOD1 recombinant monoclonal antibody, clone 3E5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 293 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: Jurkat whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: HepG2 whole cell lysate; Lane 7: U87 whole cell lysate.
Flow Cytometry
Overlay histogram showing Jurkat cells stained with SOD1 recombinant monoclonal antibody, clone 3E5 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SOD1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human SOD1.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 293 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: Jurkat whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: HepG2 whole cell lysate; Lane 7: U87 whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Jurkat cells stained with SOD1 recombinant monoclonal antibody, clone 3E5 (red line) at 1:50. -
Gene Info — SOD1
Entrez GeneID
6647Protein Accession#
P00441Gene Name
SOD1
Gene Alias
ALS, ALS1, IPOA, SOD, homodimer
Gene Description
superoxide dismutase 1, soluble
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations
Cu /Zn superoxide dismutase|Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
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Interactome
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Pathway
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Disease
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