POU5F1 monoclonal antibody, clone 7E7
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide corresponding to human POU5F1.
Host
Mouse
Theoretical MW (kDa)
45
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using POU5F1 monoclonal antobody, clone 7E7 (Cat # MAB10378) against NTERA-2 cell extracts.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells using POU5F1 monoclonal antobody, clone 7E7 (Cat # MAB10378) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using POU5F1 monoclonal antobody, clone 7E7 (Cat # MAB10378) (green) and negative control (purple). -
Gene Info — POU5F1
Entrez GeneID
5460Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
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Interactome
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Disease
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