AURKA monoclonal antibody (M01), clone 5F8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant STK6.
Immunogen
AURKA (NP_940835, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRIPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (64); Rat (63)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
STK6 monoclonal antibody (M01), clone 5F8 Western Blot analysis of STK6 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to AURKA on formalin-fixed paraffin-embedded human prostate. [antibody concentration 6 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STK6 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to STK6 on HeLa cell. [antibody concentration 30 ug/ml] -
Gene Info — AURKA
Entrez GeneID
6790GeneBank Accession#
NM_198433Protein Accession#
NP_940835Gene Name
AURKA
Gene Alias
AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
Gene Description
aurora kinase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
IPL1-related kinase|OTTHUMP00000031340|OTTHUMP00000031342|OTTHUMP00000031343|OTTHUMP00000031344|OTTHUMP00000031345|OTTHUMP00000166071|aurora-A|aurora-related kinase 1|aurora/IPL1-like kinase|breast-tumor-amplified kinase|serine/threonine kinase 15|serine/
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Interactome
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Disease
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Publication Reference
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KIBRA Protein Phosphorylation Is Regulated by Mitotic Kinase Aurora and Protein Phosphatase 1.
Xiao L, Chen Y, Ji M, Volle DJ, Lewis RE, Tsai MY, Dong J.
The Journal of Biological Chemistry 2011 Oct; 286(42):36304.
Application:WB-Ce, WB-Tr, Human, HeLa, HEK293T cells.
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Aurora Kinase A expression is associated with lung cancer histological-subtypes and with tumor de-differentiation.
Lo Iacono M, Monica V, Saviozzi S, Ceppi P, Bracco E, Papotti M, Scagliotti GV.
Journal of Translational Medicine 2011 Jun; 9:100.
Application:IHC-P, Human, Lung cancer.
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KIBRA Protein Phosphorylation Is Regulated by Mitotic Kinase Aurora and Protein Phosphatase 1.
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