AURKA recombinant monoclonal antibody, clone 1H8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AURKA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human AURKA.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescent staining of Hela cells with AURKA recombinant monoclonal antibody, clone 1H8 (Cat # RAB04389) (diluated at 1:50). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AURKA
Entrez GeneID
6790Protein Accession#
O14965Gene Name
AURKA
Gene Alias
AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
Gene Description
aurora kinase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
IPL1-related kinase|OTTHUMP00000031340|OTTHUMP00000031342|OTTHUMP00000031343|OTTHUMP00000031344|OTTHUMP00000031345|OTTHUMP00000166071|aurora-A|aurora-related kinase 1|aurora/IPL1-like kinase|breast-tumor-amplified kinase|serine/threonine kinase 15|serine/
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Interactome
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Disease
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