CAMK2D monoclonal antibody (M02), clone 1A8
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant CAMK2D.
Immunogen
CAMK2D (AAH32784, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPI
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2b Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.
Lane 1: CAMK2D transfected lysate(54.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK2D monoclonal antibody (M02) clone 1A8 (Cat # H00000817-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to CAMK2D on NIH/3T3 cell. [antibody concentration 10 ug/ml] -
Gene Info — CAMK2D
Entrez GeneID
817GeneBank Accession#
BC032784Protein Accession#
AAH32784Gene Name
CAMK2D
Gene Alias
CAMKD, DKFZp686G23119, DKFZp686I2288, MGC44911
Gene Description
calcium/calmodulin-dependent protein kinase II delta
Omim ID
607708Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns
Other Designations
CaM kinase II delta subunit|CaM-kinase II delta chain|CaMK-II delta subunit|OTTHUMP00000163829|OTTHUMP00000163830|calcium/calmodulin-dependent protein kinase (CaM kinase) II delta|calcium/calmodulin-dependent protein kinase type II delta chain
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Interactomes
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Pathways
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Diseases
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