Precise physical interactions under microscopic level turn out to be reachable because of the aid from micromanipulator. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contact with target cell for purposes like single cell collection, injection, patch clamping, etc. Here we present you step by step of probe preparation including how to do glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.
