PCYT1A recombinant monoclonal antibody, clone 4F2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 whole cell lysate with PCYT1A recombinant monoclonal antibody, clone 4F2 (Cat # RAB07523).
Immunofluorescence
Immunofluorescent staining of Hela Cells with PCYT1A recombinant monoclonal antibody, clone 4F2 (Cat # RAB07523). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PCYT1A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PCYT1A.
Theoretical MW (kDa)
42
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 whole cell lysate with PCYT1A recombinant monoclonal antibody, clone 4F2 (Cat # RAB07523).Immunofluorescence
Immunofluorescent staining of Hela Cells with PCYT1A recombinant monoclonal antibody, clone 4F2 (Cat # RAB07523). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PCYT1A
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Interactome
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Pathway
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Disease
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