MAOB recombinant monoclonal antibody, clone 9B6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of L02 whole cell lysate, HepG2 whole cell lysate with MAOB recombinant monoclonal antibody, clone 9B6 (Cat # RAB07479).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using MAOB recombinant monoclonal antibody, clone 9B6 (Cat # RAB07479) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAOB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MAOB.
Theoretical MW (kDa)
Calculated MW: 59, 4
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of L02 whole cell lysate, HepG2 whole cell lysate with MAOB recombinant monoclonal antibody, clone 9B6 (Cat # RAB07479).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using MAOB recombinant monoclonal antibody, clone 9B6 (Cat # RAB07479) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAOB
Entrez GeneID
4129Protein Accession#
P27338Gene Name
MAOB
Gene Alias
MGC26382
Gene Description
monoamine oxidase B
Omim ID
309860Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq
Other Designations
MAO, brain|MAO, platelet|adrenalin oxidase|tyramine oxidase
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Interactome
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Pathway
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Disease
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