OGA recombinant monoclonal antibody, clone 8D12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate with OGA recombinant monoclonal antibody, clone 8D12 (Cat # RAB07473).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human OGA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human OGA.
Theoretical MW (kDa)
Calculated MW: 103,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate with OGA recombinant monoclonal antibody, clone 8D12 (Cat # RAB07473).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MGEA5
Entrez GeneID
10724Protein Accession#
O60502Gene Name
MGEA5
Gene Alias
FLJ11229, FLJ23355, KIAA0679, MEA5, NCOAT, OGA
Gene Description
meningioma expressed antigen 5 (hyaluronidase)
Omim ID
604039Gene Ontology
HyperlinkGene Summary
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
Other Designations
O-GlcNAcase|OTTHUMP00000020345|hyaluronidase in meningioma|nuclear cytoplasmic O-GlcNAcase and acetyltransferase
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Interactome
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Disease
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