MGEA5 monoclonal antibody (M02), clone 1C7
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More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MGEA5 is 0.3 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGEA5 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.08 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MGEA5.
Immunogen
MGEA5 (NP_036347, 823 a.a. ~ 916 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SFHEEQEVLPETFLANFPSLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVVILGRSL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.08 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MGEA5 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MGEA5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MGEA5
Entrez GeneID
10724GeneBank Accession#
NM_012215Protein Accession#
NP_036347Gene Name
MGEA5
Gene Alias
FLJ11229, FLJ23355, KIAA0679, MEA5, NCOAT, OGA
Gene Description
meningioma expressed antigen 5 (hyaluronidase)
Omim ID
604039Gene Ontology
HyperlinkGene Summary
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM
Other Designations
O-GlcNAcase|OTTHUMP00000020345|hyaluronidase in meningioma|nuclear cytoplasmic O-GlcNAcase and acetyltransferase
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Interactome
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Disease
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