NONO recombinant monoclonal antibody, clone 8C11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate with NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer using NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence
Immunofluorescent staining of Hela Cells with NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NONO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NONO.
Theoretical MW (kDa)
Calculated MW: 55, 4
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate with NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer using NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunofluorescence
Immunofluorescent staining of Hela Cells with NONO recombinant monoclonal antibody, clone 8C11 (Cat # RAB07472). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — NONO
Entrez GeneID
4841Protein Accession#
Q15233Gene Name
NONO
Gene Alias
NMT55, NRB54, P54, P54NRB
Gene Description
non-POU domain containing, octamer-binding
Omim ID
300084Gene Ontology
HyperlinkGene Summary
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq
Other Designations
Nuclear RNA-binding protein, 54-kD|OTTHUMP00000023506|OTTHUMP00000023507|OTTHUMP00000023508|non-POU-domain-containing, octamer-binding|non-Pou domain-containing octamer (ATGCAAAT) binding protein
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com