LTF recombinant monoclonal antibody, clone 6B6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LTF.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human LTF.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using LTF recombinant monoclonal antibody, clone 6B6 (Cat # RAB07455) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LTF
Entrez GeneID
4057Protein Accession#
P02788Gene Name
LTF
Gene Alias
GIG12, HLF2, LF
Gene Description
lactotransferrin
Omim ID
150210Gene Ontology
HyperlinkGene Summary
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. [provided by RefSeq
Other Designations
growth-inhibiting protein 12|lactoferrin|neutrophil lactoferrin|talalactoferrin
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Interactome
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Disease
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