SLC16A1 recombinant monoclonal antibody, clone 9D12
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SLC16A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SLC16A1.
Theoretical MW (kDa)
Calculated MW: 54, 4
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate with SLC16A1 recombinant monoclonal antibody, clone 9D12 (Cat # RAB07401).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer using SLC16A1 recombinant monoclonal antibody, clone 9D12 (Cat # RAB07401) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SLC16A1
Entrez GeneID
6566Protein Accession#
P53985Gene Name
SLC16A1
Gene Alias
FLJ36745, HHF7, MCT, MCT1, MGC44475
Gene Description
solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
Gene Ontology
HyperlinkGene Summary
The SLC16A1 gene encodes a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT (Garcia et al., 1994 [PubMed 8124722]).[supplied by OMIM
Other Designations
OTTHUMP00000013960|monocarboxylate transporter 1|solute carrier family 16 (monocarboxylic acid transporters), member 1|solute carrier family 16, member 1
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Interactome
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