NXF1 recombinant monoclonal antibody, clone R05-1C3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 lysates with NXF1 recombinant monoclonal antibody, clone R05-1C3 (Cat # RAB04912).
Immunocytochemistry
Immunocytochemical staining of Jurkat using NXF1 recombinant monoclonal antibody, clone R05-1C3 (Cat # RAB04912)(green), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NXF1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NXF1.
Theoretical MW (kDa)
70
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunohistochemistry (Frozen sections) (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 lysates with NXF1 recombinant monoclonal antibody, clone R05-1C3 (Cat # RAB04912).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of Jurkat using NXF1 recombinant monoclonal antibody, clone R05-1C3 (Cat # RAB04912)(green), and DAPI(blue).Immunofluorescence
Immunoprecipitation
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Gene Info — NXF1
Entrez GeneID
10482Protein Accession#
Q9UBU9Gene Name
NXF1
Gene Alias
DKFZp667O0311, MEX67, TAP
Gene Description
nuclear RNA export factor 1
Omim ID
602647Gene Ontology
HyperlinkGene Summary
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
tip associating protein
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Interactome
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