PRDX3 recombinant monoclonal antibody, clone R07-3I3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate and Lane 2: HL-60 whole cell lysate with PRDX3 recombinant monoclonal antibody, clone R07-3I3 (Cat # RAB04473).
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with PRDX3 recombinant monoclonal antibody, clone R07-3I3 (Cat # RAB04473) (green). DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRDX3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PRDX3.
Theoretical MW (kDa)
Calculated MW: 28 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, 150mM NaCl, pH 7.4 (40% glycerol, 0.05% BSA and 0.01% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate and Lane 2: HL-60 whole cell lysate with PRDX3 recombinant monoclonal antibody, clone R07-3I3 (Cat # RAB04473).Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with PRDX3 recombinant monoclonal antibody, clone R07-3I3 (Cat # RAB04473) (green). DAPI was stained the cell nucleus (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — PRDX3
Entrez GeneID
10935Protein Accession#
P30048Gene Name
PRDX3
Gene Alias
AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22
Gene Description
peroxiredoxin 3
Omim ID
604769Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020590|antioxidant protein 1|thioredoxin-dependent peroxide reductase
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Interactome
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Disease
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