PRDX3 monoclonal antibody (M01), clone 1E3-B2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody (M01), clone 1E3-B2.
Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRDX3 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRDX3 is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PRDX3 on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PRDX3.
Immunogen
PRDX3 (AAH02685, 1 a.a. ~ 256 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody (M01), clone 1E3-B2.
Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRDX3 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRDX3 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PRDX3 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PRDX3
Entrez GeneID
10935GeneBank Accession#
BC002685Protein Accession#
AAH02685Gene Name
PRDX3
Gene Alias
AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22
Gene Description
peroxiredoxin 3
Omim ID
604769Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000020590|antioxidant protein 1|thioredoxin-dependent peroxide reductase
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Interactome
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Disease
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